Plant tissue culture technology has been nearly a hundred years old, and research on the industrialization of important economic plants through tissue culture technology is in the ascendant. As an important environmental factor in plant growth and development, light is also one of the main energy sources for plant growth. The use of artificial light source to directly supplement the plant in the tissue culture room is an effective way to promote plant growth, and the theoretical basis for energy-efficient plant light supplementation is mainly the selective absorption of light by plants. In recent years, efforts have been made to simulate the absorption spectrum of plants in order to develop a light source that maximizes its emission spectrum close to the absorption spectrum of the plant to produce resonance absorption, which promotes efficient photosynthesis. Improvements in plant tissue culture artificial light sources have focused on the development of lower heat dissipation and higher efficiency artificial light sources to reduce costs and meet plant growth requirements. At present, the artificial light sources used in plant tissue culture are mainly incandescent lamps, fluorescent lamps, sodium lamps, high-pressure mercury lamps, etc., but the emission spectra of these artificial light sources do not satisfactorily meet the selective requirements of plant growth for light, and the fill light efficiency is low. The life and luminous efficiency of the lamp are not ideal. The lamp heating requires a large amount of power consumption, so that the overall power consumption cost is high, and the energy consumption cost accounts for 50% to 60% of the total operating cost. Compared with the traditional artificial light source, the LED has the characteristics of adjustable light intensity and spectrum, low cooling load, high electro-optical conversion efficiency, small volume, long life, use of direct current and can set specific wavelength, fixed wavelength, etc., and has good environmental protection performance. It is a very suitable artificial light source for plant tissue culture. The use of red and blue high-brightness LEDs to create an artificial light source that adjusts the amount of light, spectrum, light frequency and duty ratio requires continuous flicker-free or high-frequency flickering. Preliminary research on the use of LED light sources in foreign and domestic countries has confirmed that the use of LED light sources as artificial light sources in greenhouses can effectively increase the growth and quality of plants. Based on LED, the plant tissue culture light source system was designed and developed. The rare medicinal plant Dendrobium candidum was used as the experimental material to verify the realization of LED-based plant tissue culture system and achieved satisfactory results. Light source system design 1, the characteristics of the LED light source system With the advancement of LED technology, its research in various fields has gradually developed. The research work of developed countries in this area started earlier, and developed countries such as the United States, Japan, the Netherlands and Russia are at the forefront of this field. In 1991, Bula first used high-brightness LEDs in space agriculture to study the application of LEDs in agriculture [4]. After Nichia invented blue LEDs in 1993, Okamoto used high-brightness red light in 1996. LED and blue LED can cultivate normal lettuce when the ratio of blue and red quantum numbers (B/R) is 1:2 [5]. From the above research results, compared with traditional light sources [9,10], LED lamps have shown incomparable in the application of artificial light sources in greenhouses, regardless of luminous efficiency, light utilization, and light controllability. The advantages. The characteristics of the LED light source system mainly include the following aspects: (1) High luminous efficiency The fluorescent lamp is a low-pressure mercury fluorescent lamp, which is a typical hot cathode arc discharge type low-pressure mercury lamp. The theoretical value of the luminous efficiency of fluorescent lamps is 20.15%, which is more than twice that of incandescent lamps. The high-pressure sodium lamp is discharged by high-pressure sodium vapor. When discharging, most of the radiant energy is concentrated on the resonance line, that is, 589.0 nm and 589.6 nm. High resonance radiation efficiency can be obtained by selecting appropriate discharge conditions. From the relative spectral energy distribution of the high-pressure sodium lamp, the peak of the emission spectrum is concentrated in the yellow light region, and the blue-violet light is rare, and there is more red light. The current luminous efficiency of LEDs can reach about 50%. (2) The utilization rate of light plants is high. Generally, less than 10% of visible light is emitted by incandescent lamps, and more than 90% is infrared rays. Therefore, the use of this light source to fill the greenhouse greenhouse crops, low efficiency, energy waste. From the perspective of plant lighting, the combination of yellow light and red light is not ideal, and it is not an ideal artificial light source. High-pressure mercury lamps emit radiation in the ultraviolet, visible, and infrared regions, and yellow-green light components in visible light account for a significant proportion. But this part of the light is not very effective for plant lighting, so it is not an ideal artificial light source. According to the research, the absorption spectrum of light by different green plants is basically the same. In the visible region, it is mainly concentrated in the blue-violet region of 400-460 nm and the red-orange region of 600-700 nm. The LED light source combined with red light and blue light can realize the plant. Maximum light utilization. (3) The controllability of light is good. The growth of organisms is studied by incandescent lamps, fluorescent lamps, sodium lamps, high-pressure mercury lamps, etc., and quantitative control cannot be prepared, and the combination of quantitative parameters cannot be arbitrarily set as needed. By using different wavelengths of LEDs, by configuring an adjustable drive control circuit, different spectral and color combinations can be selected according to different plant characteristics to obtain the light source required by the plant, and the energy consumption and cost are lower than the conventional electric light source. (4) The lamp life of the lamp is generally not less than 3000 hours, and the condition is that it is continuously ignited for 3 hours every time. If the ignition is only 1 hour, the life of the lamp is shortened to less than 70%. Therefore, when using the fluorescent lamp, try to avoid unreasonable frequent start. The LED products are extremely stable, not easy to damage, and reduce the cost of purchasing new products. The continuous operation of millions of switching operations will not damage the light source; and the energy-saving lamps will be damaged in 5000 switching operations. As can be seen from the above comparison, LED lamps show excellent performance characteristics in the application of artificial light sources for plant cultivation. 24V Lithium Battery Charger,Lithium Polymer Battery Charger,24V Bike Lithium Battery Charger,24V Bicycle Lithium Battery Charger Changxing Deli Technology Co., Ltd. , https://www.delipowers.com