Nitrate reduction experimental reagent analysis method - Database & Sql Blog Articles

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Product introduction

:

Enterobacteriaceae bacteria can reduce nitrate to nitrite, and nitrate reduction involves two processes:

1

In synthesis

During the process, the nitrate is reduced to nitrite and ammonia, and then the amino acid and other nitrogen-containing compounds in the cell are converted by ammonia;

In the metabolic process, nitrate or nitrite replaces oxygen as the terminal hydrogen acceptor in the respiratory enzyme system. Reducing nitrate

Bacteria obtain oxygen from nitrates to form nitrites and other reducing products.

Nitrate reduction experimental reagent mainly consists of p-aminobenzenesulfonic acid solution and α

-

Naphthylamine solution composition, mainly used for

Identification of Enterobacter, Pseudomonas, and the like. Bacteria such as Escherichia coli can only reduce nitrate to nitrite, nitrite and B.

The acid reacts to form nitrous acid, and the nitrite reacts with p-aminobenzenesulfonic acid in the reagent to form diazobenzenesulfonic acid, the latter and α.

-

Naphthylamine combines to form a red product; bacteria such as Pseudomonas and Serratia can reduce nitrate or nitrite to nitrogen or nitrogen oxide.

This effect is called denitrification or denitrification, which produces bubbles but no red.

product composition:

Reagent

(A):

P-aminobenzenesulfonic acid solution

50ml

RT

Protected from light

Reagent

(B):

α

-

Naphthylamine solution

50ml

RT

Protected from light

Reagent

(C):

Zinc powder

5g

RT

user's Guide

1

Share

Bring your own materials:

1

,

Nitrate medium

2

,

Constant temperature incubator

3

,

test tube

Steps

(

for reference only

)

:

1

,

The tested bacteria are inoculated into a nitrate medium.

35

°C culture

1

~

4

day.

2

,

Take an appropriate amount of p-aminobenzenesulfonic acid solution and α

-

The naphthylamine solution is an equivalent amount of nitrate reducing experimental reagent.

3

,

take

0.1ml

The nitrate reduction experimental reagent was added to the medium and the results were observed immediately.

4

,

If the experiment is negative, add a little zinc powder to the tube and observe the results immediately.

Dyeing results:

No added zinc powder is red

Positive

(

Enterobacteriaceae, Weirong

)

Add zinc powder in red

negative

Add zinc powder without discoloration

False negative

(

Pseudomonas, Serratia, etc.

)

Note: There is no color reaction when adding reagents. Possible reasons:

1

Nitrate was not reduced, the experiment was negative;

2

Nitrate is reduced to other substances such as ammonia and nitrogen, causing false negatives. At this time, a little zinc powder can be added. If it is red, the experiment is negative. If it is not red, the experiment is false negative.

Precautions:

1

,

If you want to check for nitrogen, add a small inverted tube to the tube to see if there are any bubbles.

2

,

Self-protection should be taken during bacterial culture and experimentation.

3

,

The culture time of the bacteria to be tested will also affect the staining. The culture time of the positive bacteria is too long, or the bacteria are dissolved, and the bacteria are often dissolved.

Sexual reaction.

4

,

Some anaerobic bacteria

(

Weirong

)

Also positive.

5

,

For your safety and health, wear a lab coat and disposable gloves.

Validity period:

12

Valid for months.

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